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دسته بندی:
داده کاوی - data mining
سال انتشار:
2018
عنوان انگلیسی مقاله:
A systematic data acquisition and mining strategy for chemical profiling of Aster tataricus rhizoma (Ziwan) by UHPLC-Q-TOF-MS and the corresponding anti-depressive activity screening
ترجمه فارسی عنوان مقاله:
به دست آوردن اطلاعات سیستماتیک و استراتژی استخراج مواد شیمیایی بررسی مشخصات Aster tataricus rhizoma (Ziwan) توسط UHPLC-Q-TOF-MS وغربالگری فعالیت ضد افسردگی
منبع:
Sciencedirect - Elsevier - Journal of Pharmaceutical and Biomedical Analysis, 154 (2018) 216-226: doi:10:1016/j:jpba:2018:03:022
نویسنده:
Yupeng Sun a,1, Li Li b,c,1, Man Liao a, Min Su b, Changchen Wan a, Lantong Zhang a,∗, Hailin Zhang b,∗
چکیده انگلیسی:
In this study, a systematic data acquisition and mining strategy aimed at the traditional Chinese medicine
(TCM)complexsystembasedonultrahigh-performanceliquidchromatographycoupledwithquadrupole
time of flight mass spectrometry (UHPLC-Q-TOF-MS) was reported. The workflow of this strategy is as
follows: First, the high resolution mass data are acquired by both data-dependent acquisition mode (DDA)
anddata-independentacquisitionmode(DIA).Thenaglobaldataminingthatcombinedtargetedandnon
targeted compound finding is applied to analyze mass spectral data. Furthermore, some assistant tools,
such as key product ions (KPIs), are employed for compound hunting and identification. The TCM Ziwan
(ZW, Aster tataricusrhizoma)wasusedtoillustratethisstrategyforthefirsttime.Inthisresearch,total131
compounds including organic acids, peptides, terpenes, steroids, flavonoids, coumarins, anthraquinones
and aldehydes were identified or tentatively characterized in ZW based on accurate mass measurements
within ±5 ppm error, and 50 of them were unambiguously confirmed by comparing standard compounds.
Afterwards, based on the traditional Chinese medical theory and the key determinants of firing patterns
of ventral tegmental area (VTA) dopamine (DA) neurons in the development of depression, the confirmed
compounds were subsequently evaluated the pharmacological effect of activity of VTA DA neurons and
anti-depressive efficacy. This research provided not only a chemical profiling for further in vivo study of
ZW, but also an efficient data acquisition and mining strategy to profile the chemical constituents and
find new bioactive substances for other TCM complex system.
Keywords: Chemical profiling ، Post-acquisition data mining ، Aster tataricus rhizoma ، UHPLC-Q-TOF-MS ، Ventral tegmental area ، Anti-depressant
قیمت: رایگان
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