دانلود مقاله انگلیسی رایگان:Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva) - 2019
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  • Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva) Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)
    Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)

    سال انتشار:

    2019


    عنوان انگلیسی مقاله:

    Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)


    ترجمه فارسی عنوان مقاله:

    Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva)


    منبع:

    Sciencedirect - Elsevier - Theriogenology, 125 (2019) 109-114: doi:10:1016/j:theriogenology:2018:10:022


    نویسنده:

    Felipe Martínez-Pastor a, b, *, Mercedes Alvarez b, c, Camino Guerra c, Cesar A. Chamorro b, c, Luis Anel-Lopez b, c, Paulino de Paz a, b, Luis Anel b, c, Manuel Alvarez-Rodríguez


    چکیده انگلیسی:

    Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol). Sperm was extended at 100 mill. spermatozoa/ml, cooled at 5 ○ C and frozen at —20 ○ C/min. Sperm quality was assessed pre and post-thawing (CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). Freez- ability was good overall, with total motility of 65.5% ± 2.4 initial and 55.8% ± 2.4 post-thawing. The ex- tenders affected the post-thaw sperm quality marginally. Whereas osmolalities and glycerol concentrations seemed not to differ, 430 mOsm/kg and 4% glycerol might be preferred. Egg yolk con- centrations only differed on sperm velocity (VCL: 84.0 ± 6.7 mm/s in 10% vs. 70.7 ± 6.2 mm/s in 15%, P < 0.05). Our results suggest a good cryotolerance of chamois epididymal spermatozoa, with a preferred extender composition of hyperosmotic buffer, glycerol in the 4% range and lower egg yolk (10% range) than other ruminants.© 2018 Elsevier Inc. All rights reserved.
    Keywords : Cantabrian chamois | Cryopreservation | Epididymal spermatozoa | Extender | Osmolality | Glycerol


    سطح: متوسط
    تعداد صفحات فایل pdf انگلیسی: 6
    حجم فایل: 192 کیلوبایت

    قیمت: رایگان


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