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دسته بندی:
شبکه های نورونی - neuron-networks
سال انتشار:
2020
عنوان انگلیسی مقاله:
The Vps52 subunit of the GARP and EARP complexes is a novel Arf6- interacting protein that negatively regulates neurite outgrowth of hippocampal neurons
ترجمه فارسی عنوان مقاله:
The Vps52 subunit of the GARP and EARP complexes is a novel Arf6- interacting protein that negatively regulates neurite outgrowth of hippocampal neurons
منبع:
Sciencedirect - Elsevier - Brain Research, 1745 (2020) 146905: doi:10:1016/j:brainres:2020:146905
نویسنده:
Kanta Ibuchi1, Masahiro Fukaya1, Tetsuro Shinohara, Yoshinobu Hara, Tomoko Shiroshima, Takeyuki Sugawara, Hiroyuki Sakagami
چکیده انگلیسی:
ADP ribosylation factor 6 (Arf6) is a small GTP-binding protein implicated in neuronal morphogenesis through
endosomal trafficking and actin remodeling. In this study, we identified Vps52, a core subunit of the Golgiassociated
retrograde protein (GARP) and endosome-associated recycling protein (EARP) complexes, as a novel
Arf6-binding protein by yeast two-hybrid screening. Vps52 interacted specifically with GTP-bound Arf6 among
the Arf family. Immunohistochemical analyses of hippocampal pyramidal cells revealed that fine punctate immunolabeling
for Vps52 was distributed throughout neuronal compartments, most densely in the cell body and
dendritic shafts, and was largely associated with trans-Golgi network and vesicular endomembranes. In cultured
hippocampal neurons, knockdown of Vps52 increased total length of axons and dendrites; these phenotypes
were completely restored by co-expression of shRNA-resistant full-length Vps52. However, co-expression of a
Vps52 mutant lacking the ability to interact with Arf6 restored only the Vps52-knockdown phenotype of the
dendritic length. The present findings suggest that Vps52 is a novel Arf6-interacting protein that regulates
neurite outgrowth in hippocampal neurons.
Keywords: Arf6 | Tethering factor | trans-Golgi network | Endosome | Neurite outgrowth | Hippocampus
قیمت: رایگان
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