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دسته بندی:
شبکه های نورونی - neuron-networks
سال انتشار:
2020
عنوان انگلیسی مقاله:
In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment
ترجمه فارسی عنوان مقاله:
سلولهای عصبی مشتق شده از سلولهای بنیادی سلولهای بنیادی بدن در شرایط in vitro تولید مثل سینکروتیک سیناپسی ناشی از توسعه عصبی
منبع:
Sciencedirect - Elsevier - Stem Cell Reports, Corrected proof: doi:10:1016/j:stemcr:2020:05:015
نویسنده:
Filip Rosa,1 Ashutosh Dhingra,2 Betu¨l Uysal,1 G. Dulini C. Mendis,3 Heidi Loeffler,1 Gina Elsen,1 Stephan Mueller,1 Niklas Schwarz,1 Melissa Castillo-Lizardo,2 Claire Cuddy,3 Felicitas Becker,1 Peter Heutink,2,4 Christopher A. Reid,3 Steven Petrou,3 Holger Lerche,1,5,* and Snezana Maljevic1,3,5
چکیده انگلیسی:
Neurons differentiated from induced pluripotent stem cells (iPSCs) typically show regular spiking and synaptic activity but lack more
complex network activity critical for brain development, such as periodic depolarizations including simultaneous involvement of glutamatergic
and GABAergic neurotransmission.We generated human iPSC-derived neurons exhibiting spontaneous oscillatory activity after
cultivation of up to 6 months, which resembles early oscillations observed in rodent neurons. This behavior was found in neurons
generated using a more ‘‘native’’ embryoid body protocol, in contrast to a ‘‘fast’’ protocol based on NGN2 overexpression. A comparison
with published data indicates that EB-derived neurons reach the maturity of neurons of the third trimester and NGN2-derived neurons of
the second trimester of human gestation. Co-culturing NGN2-derived neurons with astrocytes only led to a partial compensation and did
not reliably induce complex network activity. Our data will help selection of the appropriate iPSC differentiation assay to address specific
questions related to neurodevelopmental disorders
قیمت: رایگان
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