دانلود مقاله انگلیسی رایگان:استفاده از بیوانفورماتیک استاندارد برای تجزیه و تحلیل امضاهای DNA قارچی اعمال شده برای پیشروی نمونه - 2020
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  • Use of standardized bioinformatics for the analysis of fungal DNA signatures applied to sample provenance Use of standardized bioinformatics for the analysis of fungal DNA signatures applied to sample provenance
    Use of standardized bioinformatics for the analysis of fungal DNA signatures applied to sample provenance

    سال انتشار:

    2020


    عنوان انگلیسی مقاله:

    Use of standardized bioinformatics for the analysis of fungal DNA signatures applied to sample provenance


    ترجمه فارسی عنوان مقاله:

    استفاده از بیوانفورماتیک استاندارد برای تجزیه و تحلیل امضاهای DNA قارچی اعمال شده برای پیشروی نمونه


    منبع:

    Sciencedirect - Elsevier - Forensic Science International, 310 (2020) 110250. doi:10.1016/j.forsciint.2020.110250


    نویسنده:

    Julia S. Allwooda,*, Noah Fiererb,c, Robert R. Dunnd, Matthew Breena, Brian J. Reiche, Eric B. Labere, Jesse Cliftone, Neal S. Granthame, Seth A. Faitha


    چکیده انگلیسی:

    The use of environmental trace material to aid criminal investigations is an ongoing field of research within forensic science. The application of environmental material thus far has focused upon a variety of different objectives relevant to forensic biology, including sample provenance (also referred to as sample attribution). The capability to predict the provenance or origin of an environmental DNA sample would be an advantageous addition to the suite of investigative tools currently available. A metabarcoding approach is often used to predict sample provenance, through the extraction and comparison of the DNA signatures found within different environmental materials, such as the bacteria within soil or fungi within dust. Such approaches are combined with bioinformatics workflows and statistical modelling, often as part of large-scale study, with less emphasis on the investigation of the adaptation of these methods to a smaller scale method for forensic use. The present work was investigating a small-scale approach as an adaptation of a larger metabarcoding study to develop a model for global sample provenance using fungal DNA signatures collected from dust swabs. This adaptation was to facilitate a standardized method for consistent, reproducible sample treatment, including bioinformatics processing and final application of resulting data to the available prediction model. To investigate this small-scale method, 76 DNA samples were treated as anonymous test samples and analyzed using the standardized process to demonstrate and evaluate processing and customized sequence data analysis. This testing included samples originating from countries previously used to train the model, samples artificially mixed to represent multiple or mixed countries, as well as outgroup samples. Positive controls were also developed to monitor laboratory processing and bioinformatics analysis. Through this evaluation we were able to demonstrate that the samples could be processed and analyzed in a consistent manner, facilitated by a relatively user-friendly bioinformatic pipeline for sequence data analysis. Such investigation into standardized analyses and application of metabarcoding data is of key importance for the future use of applied microbiology in forensic science.
    Keywords: Forensic microbiology | Bioinformatics | Metabarcoding | Sample provenance


    سطح: متوسط
    تعداد صفحات فایل pdf انگلیسی: 9
    حجم فایل: 322 کیلوبایت

    قیمت: رایگان


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