دانلود و نمایش مقالات مرتبط با Creatinine::صفحه 1
بلافاصله پس از پرداخت دانلود کنید
نتیجه جستجو - Creatinine

تعداد مقالات یافته شده: 5
ردیف عنوان نوع
1 Colorimetric point-of-care paper-based sensors for urinary creatinine with smartphone readout
حسگرهای رنگی سنجی مبتنی بر کاغذ برای مراقبت از کراتینین ادراری با بازخوانی گوشی های هوشمند-2021
Creatinine is a clinically significant analyte used to diagnose kidney condition. However, the literature still lacks in creatinine sensors fulfilling point-of-care testing requirements. In this paper, we have developed colorimetric paper-based creatinine sensors adhering to point-of-care testing principles. The signal readout is accomplished with a smartphone modified with 3D-printed elements and processed with a self-written application compromising computer vision algorithm for automatic detection of the colored zone. Two colorimetric methods –routinely used Jaffe method and an alternative one with 3,5-dinitrobenzoic acid were both tested and compared. Hue channel intensity from HSV color space and green channel from RGB color space was used as the analytical signal in Jaffe and 3,5-dinitrobenzoate method, respectively. For both kinds of sensors the linear range of the response covered the range significant for urinary analysis, with precision, expressed as RSD, below 5%. Limit of quantification for Jaffe method was 1.05 mmol⋅ L—1 whereas it was 0.82 mmol⋅L—1 for 3,5-dinitrobenzoate method. The utility of the developed sensors to selectively quantify creatinine in undiluted urine was proved using artificial urine samples and the obtained recoveries were in the range from 70 to 129 %.
Keywords: Creatinine | Jaffe | 3,5-dinitrobenzoic acid | Microfluidic paper-based device | Smartphone | Computer vision
مقاله انگلیسی
2 Integrated metallomic and metabolomic profiling of plasma and tissues provides deep insights into the protective effect of raw and salt-processed Achyranthes bidentata Blume extract in ovariectomia rats
پروفایل فلزی و متابولیکی یکپارچه پلاسما و بافتها ، بینش عمیقی در مورد اثر محافظت شده از عصاره خام و نمک فرآوری شده Ashyranthes bidentata Blume در تخمدان موش -2019
Ethnopharmacological relevance: Achyranthes bidentata Blume (AB) is a well-known traditional Chinese medicine for treating osteoporosis and bone fracture. In the current, researches on pharmacological mechanism of AB mostly focused on molecular pathways, knowledge about its metabolic signatures is largely unclear. This study aims to develop an integrative metabolomics and metallomic approach for deciphering the biochemical basis of anti-osteoporosis effects of raw and salt-processed AB. Method: Gas chromatography–mass spectrometry (GC–MS) and inductively coupled plasma mass spectrometry (ICP-MS) were combined for metabolomic and metallomic profiling of rats serum, liver and kidney derived from the sham group, model group, E2, raw and salt-processed AB treated groups. Meanwhile, micro-CT and biomechanical analysis were carried out to ensure the success of the osteoporosis model and to validate the antiosteoporosis effect of raw and salt-processed AB. Partial least squares discriminant analysis (PLS-DA) was employed to screen potential biomarkers and the MetaboAnalyst and KEGG PATHWAY Database were used to investigate the metabolic pathway. Results: Raw and salt-processed AB protected the rats against osteoporosis, as evidenced by the restoration of the alkaline phosphatase activity, osteocalcin concentration, urine calcium/creatinine ratio and urine phosphorus/ creatinine ratio. The combination of PCA and PLS-DA revealed deviations in ninety-four differential biomarkers between raw AB treated group and model group. The identified biomarkers were primarily engaged in the metabolic pathways including galactose metabolism, urea cycle, arginine and proline metabolism, alanine metabolism, lactose degradation, ammonia recycling and glycine and serine metabolism. The levels of these biomarkers showed significant alterations and a tendency to be restored to normal values in raw and saltprocessed AB treated osteoporosis rats. Of note, the levels of trace elements, such as Zn, Se, Mn, Cu and Fe, were elevated after raw and salt-processed AB treatment. Finally, a correlation network diagram was constructed to show the biomarkers perturbed by raw and salt-processed AB. Conclusion: Our findings indicate that raw and salt-processed AB has positive effects on osteoporosis rats. Meanwhile, metabolomic and metallomic method coupled with metabolites enrichment analysis and pattern recognition serves as a useful tool for revealing the action mechanism of traditional Chinese medicine.
Keywords: Anti-osteoporosis | Achyranthes bidentata Blume | GC-MS | ICP-MS | Pattern recognition
مقاله انگلیسی
3 Droplet vitrification versus straw cryopreservation for spermatozoa banking in Persian sturgeon (Acipenser persicus) from metabolite point of view
انجماد قطره ای در مقابل انجماد کاه برای بانکی اسپرماتوزوا در ماهیان خاویاری فارسی (Acipenser persicus) از دیدگاه متابولیت-2019
Persian sturgeon (Acipenser persicus), a commercially valuable and critically endangered fish species has been suffering considerable declines in populations in the nature due to over-fishing, habitat destruction and marine pollution during past decades. Since there were no achievements in artificial reproduction programs, genetic resource banking such as gametes and embryo cryopreservation can be a good strategy however, reported resulting gamete qualities were considerably low. In the present study, the metabolome content of Persian sturgeon spermatozoa was investigated during common straw cryopreservation and novel droplet vitrification by the use of 1H NMR (Nuclear Magnetic Resonance) spectroscopy. Univariate (ANOVA) and multivariate (PCA) analysis showed significant differences in the metabolic profiles between cryopreserved and fresh spermatozoa samples. Adenine, creatine, creatine phosphate, glucose, guanidoacetate, lactate, N, N-dimethylglycine, and glycine levels showed no significant differences between these two cryopreservation techniques suggesting these metabolites and their corresponding enzymes and chemical pathways are so vulnerable to the temperature changes and even higher cooling rate in droplet vitrification could not conserve them. However, significant differences were found in acetate, creatinine, betaine, b-alanine and trimethylamine N-oxide suggesting better efficiency of droplet vitrification in protection of some metabolites associated to spermatozoa energetics, redox balance and hypoxia compensation compared to straw cryopreservation.
Keywords : Cryopreservation | Vitrification | Persian sturgeon | Spermatozoa | 1H NMR spectroscopy
مقاله انگلیسی
4 Metabolomics meets machine learning: Longitudinal metabolite profiling in serum of normal versus overconditioned cows and pathway analysis
متابولومیک با یادگیری ماشینی ملاقات می کند: پروفایل متابولیت طولی در سرم گاوهای معمولی در مقابل گاوهای بدون شرط و تجزیه و تحلیل مسیر-2019
This study aimed to investigate the differences in the metabolic profiles in serum of dairy cows that were normal or overconditioned when dried off for elucidating the pathophysiological reasons for the increased health disturbances commonly associated with overconditioning. Fifteen weeks antepartum, 38 multiparous Holstein cows were allocated to either a high body condition (HBCS; n = 19) group or a normal body condition (NBCS; n = 19) group and were fed different diets until dry-off to amplify the difference. The groups were also stratified for comparable milk yields (NBCS: 10,361 ± 302 kg; HBCS: 10,315 ± 437 kg; mean ± standard deviation). At dry-off, the cows in the NBCS group (parity: 2.42 ± 1.84; body weight: 665 ± 64 kg) had a body condition score (BCS) <3.5 and backfat thickness (BFT) <1.2 cm, whereas the HBCS cows (parity: 3.37 ± 1.67; body weight: 720 ± 57 kg) had BCS >3.75 and BFT >1.4 cm. During the dry period and the subsequent lactation, both groups were fed identical diets but maintained the BCS and BFT differences. A targeted metabolomics (AbsoluteIDQ p180 kit, Biocrates Life Sciences AG, Innsbruck, Austria) approach was performed in serum samples collected on d −49, +3, +21, and +84 relative to calving for identifying and quantifying up to 188 metabolites from 6 different compound classes (acylcarnitines, AA, biogenic amines, glycerophospholipids, sphingolipids, and hexoses). The concentrations of 170 metabolites were above the limit of detection and could thus be used in this study. We used various machine learning (ML) algorithms (e.g., sequential minimal optimization, random forest, alternating decision tree, and naïve Bayes–updatable) to analyze the metabolome data sets. The performance of each algorithm was evaluated by a leave-one-out crossvalidation method. The accuracy of classification by the ML algorithms was lowest on d 3 compared with the other time points. Various ML methods (partial least squares discriminant analysis, random forest, information gain ranking) were then performed to identify those metabolites that were contributing most significantly to discriminating the groups. On d 21 after parturition, 12 metabolites (acetylcarnitine, hexadecanoyl-carnitine, hydroxyhexadecenoyl-carnitine, octadecanoyl-carnitine, octadecenoyl-carnitine, hydroxybutyryl-carnitine, glycine, leucine, phosphatidylcholine-diacyl-C40:3, trans-4-hydroxyproline, carnosine, and creatinine) were identified in this way. Pathway enrichment analysis showed that branched-chain AA degradation (before calving) and mitochondrial β-oxidation of long-chain fatty acids along with fatty acid metabolism, purine metabolism, and alanine metabolism (after calving) were significantly enriched in HBCS compared with NBCS cows. Our results deepen the insights into the phenotype related to overconditioning from the preceding lactation and the pathophysiological sequelae such as increased lipolysis and ketogenesis and decreased feed intake
Key words: metabolomics | machine learning | metabolic pathway | transition cow
مقاله انگلیسی
5 تأثیر ماده همبند سمی بر عملکرد تولید گاوهای شیرده فریزیانی
سال انتشار: 2019 - تعداد صفحات فایل pdf انگلیسی: 9 - تعداد صفحات فایل doc فارسی: 25
از 20 گاو شیرده فریزیانی با متوسط وزن 540±17.59 کیلوگرم و در دوره شیردهی دوم تا پنجم، 60 روز قبل از زایمان استفاده شد و تا 120 روز آزمایش تغذیه شیردهی ادامه یافت تا تأثیر ماده سمی (بنتونیت و زئولیت) روی عملکرد تولید گاوهای شیرده بررسی شود. گاوها به طور تصادفی به چهار گروه مشابه تقسیم شدند (3 نفر). همه گاوها جیره اساسی (BR) دریافت کردند که متشکل از مخلوط علوفه کنسانتره (CFM) ، سیلاژ ذرت (CS) و کاه برنج (RS) بود. گروه 1 (BR) جیره اساسی بدون مکمل دریافت کرده و به عنوان جیره شاهد در نظر گرفته شد، در حالی که گروه‌های 2، 3 و 4 به ترتیب رژیم شاهد را به علاوه 2% بنتونیت ، 1% بنتونیت به علاوه 1٪ زئولیت یا 2٪ زئولیت مصرف DM به عنوان جیره‌های آزمایشی دریافت کردند. نتایج نشان داد که گروه‌های‌ دریافت کننده مکمل (P<0.05) نسبت به گروه‌هایی که مکمل دریافت نکردند، مصرف کل DM ، TDN و DCP بیشتری داشتند. همچنین با همین روند، غلظت pH و TVFA به طور قابل توجهی افزایش یافت (P<0.05)، در حالی که آمونیاک - N در گروه‌های‌ دریافت کننده مکمل نسبت به گروه 1 به طور چشمگیری (P<0.05) کاهش یافت. گروه 2 بیشترین غلظت پروتئین کل، گلوبولین ، گلوکز و T3 را (P<0.05) ثبت کرد و پس از آن گروه 3 و گروه 4 قرار گرفتتند، در حالی که گروه 1 کمترین مقادیر را داشت. در حالی که غلظت آلبومین با افزودن بنتونیت و زئولیت به طور چشمگیری کاهش یافت (P<0.05). غلظت کراتینین، اوره ، چربی کل و کلسیم و همچنین فعالیت AST و ALT برای گروه های مختلف تقریباً مشابه بود. تولید شیر واقعی و 4٪ FCM در گروه 2 (P<0.05) نسبت به گروه 4 و گروه 1 به طور چشمگیری بیشتر بود و از جیره گروه 3 بیشتر نبود: گروه 2 (P<0.05) بیشترین مقدار چربی ، پروتئین، لاکتوز، SNF و TS را نشان داد، در حالی که گروه 1 کمترین مقادیر را داشت. مقدار خاکستر شیر برای گروه های مختلف تقریباً مشابه بود. مکمل بنتونیت و زئولیت ضریب تبدیل غذایی را بهبود بخشید و گروه 2 بهترین مورد را ثبت کرد. این اختلاف تنها بین جیره بنتونیت (گروه 2) و شاهد (گروه 1) قابل توجه بود. گروه 2 بیشترین هزینه علوفه روزانه ، تولید محصول 4٪ FCM، درآمد خالص و بازده اقتصادی را ثبت کرد و پس از آن گروه 3 و گروه 4 بیشترین مقدار را داشتند، در حالی که گروه 1 کمترین هزینه علوفه، درآمد خالص و بازده اقتصادی را داشت، هزینه علوفه هر کیلوگرم 4٪ FCM گروه 2 و گروه 3 و گروه 4 به طور قابل توجهی کمتر بود (P<0.05)، در حالی که گروه 1 بیشترین مقدار را داشت. در نتیجه، درمقایسه با سایر جیره های تکمیلی و شاهد (بدون مکمل)، مکمل بنتونیت برای گاوهای شیرده فریزیانی در سطح 2٪ مصرف DM، به عنوان ماده همبند سمی بر هضم‌پذیری، لیکور شکمبه، برخی از پارامترهای خونی، مصرف علوفه ، میزان تولید و ترکیب شیر، ضریب تبدیل غذا یی و بازده اقتصادی بهترین اثر مثبت را داشت.
کلمات کلیدی: ماده همبند سمی گاوها | هضم‌پذیری | پارامترهای شکمبه و خون | عملکرد تولیدی و بازده اقتصادی.
مقاله ترجمه شده
rss مقالات ترجمه شده rss مقالات انگلیسی rss کتاب های انگلیسی rss مقالات آموزشی
logo-samandehi