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A self-assembled layer-by-layer surface modification to fabricate the neuron-rich model from neural stem/precursor cells
اصلاح سطح لایه به لایه خود مونتاژ برای ساخت مدل غنی از نورون از سلولهای بنیادی / پیش ساز عصبی-2020 Background/Purpose: In vitro neural cell-based models have been widely used to mimic the
in vivo neural tissue environments and quantitatively understand the effects of pharmaceutical
molecules on neural diseases. Recently, several biomimetic neural tissue models have
been widely developed by using biomaterials or surface modification. However, the complex
protocols of material synthesis or surface modification lack an easy execution to fabricate
the neuron favorite environment.
Methods: In this study, we utilized a layer-by-layer technique as a surface modification method
for regulating the behaviors of neural stem/precursor cells (NSPCs) on material surfaces. Polyelectrolyte
multilayers (PEMs) via alternate deposition of poly (allylamine hydrochloride) (PAH)
and poly (sodium-4-styrenesulfonate) (PSS) were used to culture NSPCs. After incubation for 7
days, the neuronal differentiation of NSPCs and synapse function of differentiated neurons
were identified by immunocytochemistry for lineage specific markers.
Results: Compared with the only PAH film, the PSS-ending film (neuron-rich model) was
shown to significantly promote differentiation of NSPCs into neurons (more than 50%), form
a neuronal network structure; and differentiated neurons exhibiting functional synaptic
activity. KEYWORDS : Polyelectrolyte multilayer (PEM) | Neural stem/ precursor cells | (NSPCs) | Neuronal | differentiation | Neurite outgrowth |
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