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نتیجه جستجو - Ovulation rate

تعداد مقالات یافته شده: 3
ردیف عنوان نوع
1 Progesterone-based timed AI protocols for Bos indicus cattle I: Evaluation of ovarian function
پروتکل های هوش مصنوعی بهنگام مبتنی بر پروژسترون برای گاو Bos indicus cattle I: ارزیابی عملکرد تخمدان-2020
Three experiments evaluated ovarian dynamics and circulating progesterone (P4) during P4-based protocols initiated with GnRH, estradiol benzoate (EB), or no additional treatment in Nelore (Bos indicus) cattle. In Exp 1 (n ¼ 59 cows), a 5-d P4-only protocol (P-5d; D0: P4 implant alone (1g); D5: P4 removal, 0.5 mg estradiol cypionate [EC], 0.526 mg cloprostenol [PGF], and 300 IU equine chorionic gonadotropin [eCG]; D7: 8.4 mg buserelin acetate [GnRH]) was compared to a 9d protocol initiated with EB (EB-9d; D0: 2 mg EB þ P4; D9: P4 removal þ EC þ PGF þ eCG), and to a 7d GnRH protocol (G-7d; D0: 16.8 mg GnRH þ P4; D6: PGF þ eCG; D7: P4 removal þ PGF; D9: GnRH). Exp 2 (n ¼ 55 cows) compared G- 7d and EB-7d protocols (similar to EB-9d, but D9 treatments were done on D7). Exp 3 (n ¼ 64 heifers) compared EB-7d, G-7d, and P-5d protocols. For all experiments, daily ovarian ultrasonography was done from D0 until 4d after implant withdrawal and blood samples were collected at D0 and first PGF. Follicle dynamics were determined for each individual animal, analyzed within individual experiments, and afterwards combined to determine overall effects of treatments. The protocol that began with GnRH, G- 7d, had greater ovulation rate after D0 with subsequently greater number of CL and circulating P4 at time of PGF (52.8%, 1.0 ± 0.1 CL, 4.0 ± 0.4 ng/mL) than for EB protocols (12.1%, 0.4 ± 0.05 CL, 2.0 ± 0.2 ng/mL), or P-5d (2.5%, 0.6 ± 0.09 CL, 2.6 ± 0.3 ng/mL). The G-7d and EB protocols had synchronized follicle wave emergence in 92.1% of animals but with distinct patterns. For the G-7d group, wave emergence occurred earlier in ovulating than non-ovulating animals (1.4 ± 0.2 d vs 2.5 ± 0.4 d). By comparison, most animals in EB-7d or EB-9d (80.3%) displayed atresia of the dominant follicle, followed by wave emergence 2e3 d after EB treatment. In contrast, P-5d protocol synchronized wave emergence in only 30.0% of cows. Nevertheless, no differences among treatments were detected for ovulation at end of the protocol (85.7%). In conclusion, the P-5d protocol did not synchronize follicle wave emergence but produced similar final ovulation, whereas, GnRH and EB protocols had follicle dynamics synchronized by distinct mechanisms that produced differences in CL number and P4 at the time of PGF treatment but similar final ovulation. Based on ovarian function, each of these synchronization methods are promising for use in FTAI, although fertility still needs to be evaluated.
Keywords: Artificial insemination | Beef cattle | Estradiol | GnRH | Synchronization
مقاله انگلیسی
2 Treatment with estradiol cypionate at progesterone withdrawal reduces handling without compromising the pregnancy rate to timed- AI in buffalo
درمان با استرادیول سیپیونات در ترک پروژسترون ، دست زدن بدون به خطر انداختن میزان حاملگی به موقع هوش مصنوعی در بوفالو را کاهش می دهد-2020
The aim of this study was to determine if treatment with estradiol cypionate (EC) at the time of P4 withdrawal induced ovulation in a synchronization/timed-AI (TAI) protocol in buffalo. In Experiment 1, 56 buffaloes received an intravaginal P4 device (1.0 g) plus estradiol benzoate (EB, 2.0 mg im) on Day 0 (D0). On Day 9, the P4 device was removed and buffaloes were given PGF2a (0.53 mg im sodium cloprostenol) plus eCG (400 IU im). Buffaloes were then randomly allocated to one of two groups: Group GEC (n ¼ 29), treated with EC (1.0 mg im) at P4 device removal; Group GEB (n ¼ 27), treated with EB (1.0 mg im) 24 h after P4 device removal. Ovarian ultrasound was undertaken on: D0, to ascertain general ovarian status; D9 to D11 (every 24 h), to measure diameter of the largest follicle (LF) and follicular growth rate; D11 to D13 (every 12 h for 72 h), to determine the time of ovulation and ovulation rate. Following P4 device removal, Groups GEC and GEB had a similar follicular growth rate (0.9 ± 0.1 and 1.1 ± 0.1 mm/day, respectively; P ¼ 0.15) and similar LF diameter on D11 (11.4 ± 0.6 and 12.5 ± 0.5 mm; P ¼ 0.12). Groups GEC and GEB also had a similar diameter of the ovulatory follicle (13.0 ± 0.5 and 13.4 ± 0.6 mm; P ¼ 0.52), interval from P4 device removal to ovulation (68.2 ± 2.8 and 71.1 ± 1.4 h; P ¼ 0.41) and ovulation rate (62.1% and 70.4%; P ¼ 0.44). In Experiment 2, 199 buffaloes were assigned to the two treatments in Experiment 1 (GEC, n ¼ 100; GEB, n ¼ 99). All animals underwent TAI 56 h after P4 device removal and pregnancy diagnosis was preformed on D41. The pregnancy rate was similar for Groups GEC and GEB (50.0 and 45.5%, respectively; P ¼ 0.45). The findings indicate that treatment with EC at the time of P4 withdrawal induces ovulation and achieves the same pregnancy rate to TAI as treatment with EB 24 h after P4 removal. The use of EC requires one less handling which is highly important in facilitating practical adoption of TAI in assisted breeding and genetic improvement in buffalo.
Keywords: Buffalo | Estradiol cypionate | Ovulation synchronization | Pregnancy | Timed-AI
مقاله انگلیسی
3 Long interval prostaglandin-based treatment regimens do not affect ovulatory or prolificacy rates of multiparous ewes after cervical fixed timed AI
رژیم های درمانی مبتنی بر پروستاگلاندین با فاصله طولانی بر میزان تخمک گذاری یا ازدیاد میش های چندشاخ بعد از هوش مصنوعی به موقع دهانه رحم تأثیر نمی گذارد-2020
To evaluate effects of a longer, than conventional, interval between time of prostaglandin F2α (PG)-based administrations in a PG-based treatment regimen for fixed timed AI (FTAI) on ovulation rate (OR), non-estrous return rate on Day 21 subsequent to the time of AI (NRR21), as well as conception, prolificacy and fecundity rates, ewes were assigned to two groups. Ewes of treatment group (PG15) were estrous-synchronized using two PG doses 15 days apart and FTAI was conducted at 56 h after the second PG administration (Day 0). Ewes of the Control group (SE) had imposed a pre-estrous synchrony treatment regimen with two PG doses 7 days apart and AI was conducted after detection of spontaneous estrus from 17 to 19 days after the second PG administration (Day 0). Ovulation rate on Day 8, NRR21, conception, prolificacy and fecundity rates on Day 60 were evaluated. There were no differences (P > 0.05) between ewes of the PG15 and SE groups in OR (1.47 ± 0.50 and 1.54 ± 0.50, respectively) or prolificacy (1.42 ± 0.80 and 1.33 ± 0.62, respectively), however, there were lesser values (P < 0.05) in the PG15 than SE group for NRR21 (65.2% and 91.3%, respectively), conception (59.8% and 91.3%, respectively) and fecundity (84.8% and 120%, respectively). The longer interval with the PG-based treatment regimen does not affect OR and prolificacy, but there is a lesser NRR21, conception and fecundity rate in comparison to ewes of the Control group.
Keywords: Prostaglandin | Fixed timed AI | Ovulation rate | Prolificacy | Conception | Ewe
مقاله انگلیسی
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