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نتیجه جستجو - خصوصیات ساختار

تعداد مقالات یافته شده: 2
ردیف عنوان نوع
1 Synchrotron-based techniques for studying the environmental health effects of heavy metals: Current status and future perspectives
تکنیک های مبتنی بر سنکروترون برای بررسی اثرات بهداشتی محیط زیست فلزات سنگین: وضعیت فعلی و دیدگاه های آینده-2020
This review summarizes the state-of-the-art synchrotron-based techniques for studying the environmental health effects of heavy metals exposure. Synchrotron radiation based X-ray fluorescence (SRXRF) is widely applied in quantification of metals in different biological and environmental samples. X-ray absorption spectrometry (XAS) is used for speciation of heavy metals. With high energy resolution fluorescence detected (HERFD) XAS, it is possible to study heavy metals in biological samples at realistic concentrations. The focused synchrotron-based X-ray is applied to image metals down to nm resolution at 2- or 3- dimension (2D or 3D). The combination of XAS with SRXRF can realize 2D or 3D spatial speciation, along with other techniques like scanning transmission X-ray microscopy (STXM) and full field XAS. The structure of metal-binding biomolecules can be characterized by Protein X-ray Diffraction (PX) and/or XAS, together with neutron scattering. The future aspects of multimode detection, new imaging methods, fast detector technologies and big data strategies in synchrotron-based techniques were also discussed.
Keywords: Synchrotron radiation | Quantification | Speciation | Mapping | Spatial speciation | Structure characterization | Heavy meta
مقاله انگلیسی
2 Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit
خصوصیات ساختاری و فعالیت سیستم ایمنی از پلی ساکارید اسید جدید جدا شده از خمیر میوه Rosa laevigata Michx-2019
A novel acid polysaccharide (PPRLMF-2) with the Mw of 137,123 Da and a triple-helix conformation was first isolated from the pulp of Rosa laevigata Michx fruit. Structural characterization showed that PPRLMF- 2 consisted of rhamnose (7.6%), arabinose (26.5%), xylose (3.5%), mannose (0.9%), glucose (5.7%), galactose (31.9%) and galacturonic acid (23.9%). The methylation and NMR (1D and 2D) analysis revealed that PPRLMF-2 contained 16 types of glycosidic linkages. The immunomodulatory activity assays indicated that PPRLMF-2 could significantly enhance phagocytosis, the secretion and mRNA expression of cytokines in RAW 264.7 cells. Additionally, SR, GR, TLR-2, and TLR-4 were the main pattern recognition receptors (PRRs) of PPRLMF-2 to upregulate the p-ERK, p-JNK, p-p38, and p-p65. These results suggested that PPRLMF-2 could recognize the PRRs of the macrophages to enhance the immunomodulatory activity via activation of the MAPKs and NF-jB signaling pathways. This study provides important implications of PPRLMF-2 as an attractive immunomodulatory functional food.
Keywords: Rosa laevigata Michx | Polysaccharide | Structural characterization | RAW264.7 cells | Immunomodulatory activity
مقاله انگلیسی
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