با سلام خدمت کاربران در صورتی که با خطای سیستم پرداخت بانکی مواجه شدید از طریق کارت به کارت (6037997535328901 بانک ملی ناصر خنجری ) مقاله خود را دریافت کنید (تا مشکل رفع گردد).
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Treatment with estradiol cypionate at progesterone withdrawal reduces handling without compromising the pregnancy rate to timed- AI in buffalo
درمان با استرادیول سیپیونات در ترک پروژسترون ، دست زدن بدون به خطر انداختن میزان حاملگی به موقع هوش مصنوعی در بوفالو را کاهش می دهد-2020 The aim of this study was to determine if treatment with estradiol cypionate (EC) at the time of P4
withdrawal induced ovulation in a synchronization/timed-AI (TAI) protocol in buffalo. In Experiment 1,
56 buffaloes received an intravaginal P4 device (1.0 g) plus estradiol benzoate (EB, 2.0 mg im) on Day
0 (D0). On Day 9, the P4 device was removed and buffaloes were given PGF2a (0.53 mg im sodium
cloprostenol) plus eCG (400 IU im). Buffaloes were then randomly allocated to one of two groups: Group
GEC (n ¼ 29), treated with EC (1.0 mg im) at P4 device removal; Group GEB (n ¼ 27), treated with EB
(1.0 mg im) 24 h after P4 device removal. Ovarian ultrasound was undertaken on: D0, to ascertain general
ovarian status; D9 to D11 (every 24 h), to measure diameter of the largest follicle (LF) and follicular
growth rate; D11 to D13 (every 12 h for 72 h), to determine the time of ovulation and ovulation rate.
Following P4 device removal, Groups GEC and GEB had a similar follicular growth rate (0.9 ± 0.1 and
1.1 ± 0.1 mm/day, respectively; P ¼ 0.15) and similar LF diameter on D11 (11.4 ± 0.6 and 12.5 ± 0.5 mm;
P ¼ 0.12). Groups GEC and GEB also had a similar diameter of the ovulatory follicle (13.0 ± 0.5 and
13.4 ± 0.6 mm; P ¼ 0.52), interval from P4 device removal to ovulation (68.2 ± 2.8 and 71.1 ± 1.4 h;
P ¼ 0.41) and ovulation rate (62.1% and 70.4%; P ¼ 0.44). In Experiment 2, 199 buffaloes were assigned to
the two treatments in Experiment 1 (GEC, n ¼ 100; GEB, n ¼ 99). All animals underwent TAI 56 h after P4
device removal and pregnancy diagnosis was preformed on D41. The pregnancy rate was similar for
Groups GEC and GEB (50.0 and 45.5%, respectively; P ¼ 0.45). The findings indicate that treatment with
EC at the time of P4 withdrawal induces ovulation and achieves the same pregnancy rate to TAI as
treatment with EB 24 h after P4 removal. The use of EC requires one less handling which is highly
important in facilitating practical adoption of TAI in assisted breeding and genetic improvement in
buffalo. Keywords: Buffalo | Estradiol cypionate | Ovulation synchronization | Pregnancy | Timed-AI |
مقاله انگلیسی |